Motivation¶
The QIIME 2 Moving Pictures tutorial is the gold standard for newcomers to get familiar with 16S rRNA analysis. While the tutorial covers the essential steps of a basic analysis, it focuses primarily on executing commands and evaluating outputs without providing a deep look into the underlying processes.
This motivated me to create this blog: to provide a high-level abstraction of the crucial workflows operating behind the scenes of QIIME 2 commands.
Content¶
This blog explores the internal logic and workflows of the QIIME 2 (v2026.1.0) commands featured in the tutorial. Starting with demultiplexing of raw sample sequences. These sequences are subsequently processed using DADA2 and Deblur, two independent denoising pipelines designed to eliminate sequencing artifacts. Finally, the outputs from DADA2 are used for Diversity analysis to analyze alpha and beta matrices.
The practiced method to complile context for this blog can be found in Methology.